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KMID : 1377020120090010010
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Tissue Engineering and Regenerative Medicine
2012 Volume.9 No. 1 p.10 ~ p.16
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Neural differentiation of rat muscle-derived stem cells in the presence of valproic acid: A preliminary study
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Kwon Jin-Seon
Kim Gyeong-Hae
Kim Da-Yeon
Lee Bit-Na
Lee Bong
Kim Jae-Ho
Min Byoung-Hyun
Kim Moon-Suk
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Abstract
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In this study, we chose valproic acid (VA) for inducing neural differentiation in rat muscle-derived stem cells (rMDSCs). After pre-induction with basic fibroblast growth factor (bFGF) and subsequent treatment with VA, VA-related cytotoxicity to rMDSCs was relatively low. In addition, VA-treated rMDSCs exhibited a neural-like morphology after 1, 4, and 7 days. The immunocytochemical results of VA-treated rMDSCs showed abundant cells stained positive for neuron-specific enolase (NSE) and neuron filament (NF) as neural markers, oligodendrocyte transcription factor 2 (Olig-2) as an oligodendrocyte marker, and glial fibrillary acidic protein (GFAP) as an astrocyte marker. Our results suggest that rMDSCs can be differentiated into a neural-like phenotype by using VA.
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KEYWORD
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rat muscle-derived stem cells, valproic acid, differentiation, neuron, glia cells
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